Qualitative and Quantitative Evaluation of Indirect Immuno-fluorescent H-2 Stain on Tissue Sections

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Qualitative and quantitative evaluation of indirect immunofluorescent H-2 stain on tissue sections.

We have utilized H-2 specific antisera from congenic resistant donor recipient combinations to localize H-2 antigens in tissue sections using the indirect immunofluorescent technique. H-2 specific immunofluorescence is observed in spleen, kidney, and liver sections: however, especially with liver sections, there are cases in which the expected specific stain is weak or absent. In addition to mi...

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Although orcein has been in use for a long time as a stain for elastic fibres, it is only recently that it has been rediscovered for the demonstration of a variety of other substances. These include hepatitis B surface antigen (HBsAg),' 2 copper-associated protein,34 and more recently, intestinal sulphated mucins.35 Sipponen5 studied the normal distribution of orceinpositive mucin in the gastro...

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ژورنال

عنوان ژورنال: Tissue Antigens

سال: 2008

ISSN: 0001-2815,1399-0039

DOI: 10.1111/j.1399-0039.1979.tb00826.x